Extraction and amplification of genomic DNA from human blood on nanoporous aluminum oxide membranes.

Evidence of lytic infection of Epstein-Barr virus (EBV) in EBV-positive gastric carcinoma.stedt K, et al. Diagnostic implications of human cytomegalovirus immediate early-1 and pp67 mRNA detection in whole-blood samples from liver transplant patients using nucleic acid sequence-based amplification. Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection. The Epstein-Barr virus candidate vaccine antigen gp340/220 is highly conserved between virus types A and B. The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein. In vivo degradation of RNA polymerase II largest subunit triggered by ␣-amanitin. Detection of circular and linear herpesvirus DNA molecules in mammalian cells by gel electrophore-sis. Limonium sinense suppresses herpes simplex virus type 1 replication in Vero cells by regulation of viral macromolecular synthesis. G, et al. Routine use of real-time quantitative PCR for laboratory diagnosis of Epstein-Barr virus infections. Posttransplantation cutaneous B-cell lymphoma with monoclonal Epstein-Barr virus infection, responding to acyclovir and reduction in immunosup-pression. WO, et al. Treatment of Epstein-Barr virus-induced posttransplantation lymphoproliferative disorder with foscarnet alone in an adult after simultaneous heart and renal transplantation.tion of early antigen BZLF1/ZEBRA protein of Epstein-Barr virus can predict the effectiveness of antiviral treatment in patients with post-transplant lymphoproliferative disease. pattern of Epstein-Barr virus and methylation status in Epstein-Barr virus-associated hemophagocytic syndrome. analysis of the expression of Epstein-Barr virus lytic genes in nasopharyn-geal carcinoma biopsies. Purification of genomic DNA from biological samples is a requisite initial step in the performance of many molecular diagnostic assays. Traditional purification methods based on phenol– chloroform extraction and ethanol precipitation have been largely supplanted by adsorption and elution procedures based on silica and coated magnetic bead matrices. In most applications, the purified genomic DNA must be physically separated from the adsorption matrix before downstream processes such as amplification by PCR. Separation is done to reduce inhib-itory effects of adsorption matrices on amplification processes , which may result from matrix interactions with enzymes and other reaction components (1). The requirement for separation necessitates the addition of a transfer step performed by either manual or robotic means. To further streamline molecular diagnostic analyses, new purification matrices are needed. Specifically, a matrix capable of purification that does not inhibit amplification chemistries would open the path to development of instrumentation that performed purification, amplification , and detection in an integrated format. We have been investigating the properties of a commercially available porous aluminum oxide membrane …